My Painful Quest for Plasmium in Silksong (And How You Can Do It Too)

So, you’ve bumped into Alchemist Zylotol in the Wormways, huh? Yeah, that weird bug with the scientific vibes. He wants Plasmium, and he’s not exactly handing out a treasure map. I remember my first run—got lost in those winding tunnels for like an hour. But fear not, fellow Hollow Knight: Silksong player. I’ve done the dirty work, and I’m here to spill the beans on the Alchemist’s Assistant quest. It’s 2026, and this side quest is still one of the best early-game rewards you can snag. Let’s dive in.

First Things First: You Need the Needle Phial

Before you go poking around for Plasmium, you gotta equip the right tool. The Needle Phial icon Needle Phial is your key to extracting that glowing blue goo. Sit on the bench right next to Zylotol (convenient, I know), pop open your Tools menu, and slot it in. No Needle Phial? No Plasmium. Simple as that.

All Plasmium bud locations for Alchemist's Assistant quest in Silksong

The Three Plasmium Locations (Don’t Get Lost, Okay?)

There are exactly three Plasmium buds scattered across the Wormways. I’ve marked them all below. Trust me, the first time I ran this, I walked right past one because I was too busy staring at a spike pit. Don’t be like me.

1. The Alcove Right Under Zylotol’s Nose

This one’s a gimme. From the Alchemist’s spot, take the exit to the right and drop down a level. Turn left, and you’ll see a spike pit. Use your Wall Climb to get above it, then go left and drop down again. Boom—blue glow in the alcove to your right. Hold that Tool activation hotkey, and the Needle Phial does its thing.

First Plasmium location route in Silksong

2. Deeper Into the Bowels of the Wormways

The second bud is further down, and the route’s a bit twisty. I’ll be honest, I fell into a pit of acid my first try. But you got this. Follow the main path downward, keep an eye out for ledges, and eventually you’ll spot the telltale blue shimmer. Here’s the sequence:

Second Plasmium location route - part 1

Second Plasmium location route - part 2

Second Plasmium location route - part 3

Second Plasmium location route - part 4

Second Plasmium location in Silksong

Once you see it, extract it with the Needle Phial. Easy peasy.

3. The Hidden Node West of the Second One

This one’s a bit sneaky. It’s in a hidden section west of the second node. You’ll need to break a wall—yes, a breakable wall. I spent ten minutes slashing random spots before I found it. Don’t be shy; just smack everything that looks suspicious.

Third Plasmium location route - part 1

Third Plasmium location route - part 2

Third Plasmium location breakable wall in Silksong

Third Plasmium location in Silksong

After you grab all three, head back to Zylotol. He’ll be thrilled—well, as thrilled as a bug scientist can be.

The Reward: Plasmium Phial (Worth Every Scratch)

Alchemist's Assistant reward - Plasmium Phial in Silksong

Zylotol hands over the Plasmium Phial icon Plasmium Phial. This bad boy temporarily boosts Hornet’s base health by three extra masks. In boss fights? Absolute lifesaver. I’m not kidding—those ex
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